Automated pipeline for purification, biophysical and x-ray analysis of biomacromolecular solutions.
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ABSTRACT:
SUBMITTER: Graewert MA
PROVIDER: S-EPMC5377070 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
Publications
Automated pipeline for purification, biophysical and x-ray analysis of biomacromolecular solutions.
Graewert Melissa A MA Franke Daniel D Jeffries Cy M CM Blanchet Clement E CE Ruskule Darja D Kuhle Katja K Flieger Antje A Schäfer Bernd B Tartsch Bernd B Meijers Rob R Svergun Dmitri I DI
Scientific reports 20150601
Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAXS characterization of the separated components. The system coupled to an automated data analysis pipeline provides a novel tool to study difficult samples at the P12 synchrotron beamline (PETRA-3, EMB ...[more]
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