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Damidseq_pipeline: an automated pipeline for processing DamID sequencing datasets

ABSTRACT: DamID is a powerful technique for identifying regions of the genome bound by a DNA-binding (or DNA-associated) protein. Currently no method exists for automatically processing next-generation sequencing DamID (DamID-seq) data, and the use of DamID-seq datasets with normalisation based on read-counts alone can lead to high background and the loss of bound signal. DamID-seq thus presents novel challenges in terms of normalisation and background minimisation. We describe here damidseq_pipeline, a software pipeline that performs automatic normalisation and background reduction on multiple DamID-seq FASTQ or BAM datasets.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE69184 | GEO | 2015/06/26

SECONDARY ACCESSION(S): PRJNA284767

REPOSITORIES: GEO

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damidseq_pipeline: an automated pipeline for processing DamID sequencing datasets

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