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A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle.

ABSTRACT: The endocrine system dynamically controls tissue differentiation and homeostasis, but has not been studied using dynamic tissue culture paradigms. Here we show that a microfluidic system supports murine ovarian follicles to produce the human 28-day menstrual cycle hormone profile, which controls human female reproductive tract and peripheral tissue dynamics in single, dual and multiple unit microfluidic platforms (Solo-MFP, Duet-MFP and Quintet-MPF, respectively). These systems simulate the in vivo female reproductive tract and the endocrine loops between organ modules for the ovary, fallopian tube, uterus, cervix and liver, with a sustained circulating flow between all tissues. The reproductive tract tissues and peripheral organs integrated into a microfluidic platform, termed EVATAR, represents a powerful new in vitro tool that allows organ-organ integration of hormonal signalling as a phenocopy of menstrual cycle and pregnancy-like endocrine loops and has great potential to be used in drug discovery and toxicology studies.

SUBMITTER: Xiao S

PROVIDER: S-EPMC5379057 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle.

Xiao Shuo S Coppeta Jonathan R JR Rogers Hunter B HB Isenberg Brett C BC Zhu Jie J Olalekan Susan A SA McKinnon Kelly E KE Dokic Danijela D Rashedi Alexandra S AS Haisenleder Daniel J DJ Malpani Saurabh S SS Arnold-Murray Chanel A CA Chen Kuanwei K Jiang Mingyang M Bai Lu L Nguyen Catherine T CT Zhang Jiyang J Laronda Monica M MM Hope Thomas J TJ Maniar Kruti P KP Pavone Mary Ellen ME Avram Michael J MJ Sefton Elizabeth C EC Getsios Spiro S Burdette Joanna E JE Kim J Julie JJ Borenstein Jeffrey T JT Woodruff Teresa K TK

Nature communications 20170328

The endocrine system dynamically controls tissue differentiation and homeostasis, but has not been studied using dynamic tissue culture paradigms. Here we show that a microfluidic system supports murine ovarian follicles to produce the human 28-day menstrual cycle hormone profile, which controls human female reproductive tract and peripheral tissue dynamics in single, dual and multiple unit microfluidic platforms (Solo-MFP, Duet-MFP and Quintet-MPF, respectively). These systems simulate the in v ...[more]

PMID: 28350383

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Project description:BackgroundThis study was undertaken to discover whether the vaginal microbe of women at childbearing age is different among groups defined by urogenital tract infections, childbearing history and menstrual cycle, respectively.ResultsThis was a multiple case-control study of women at childbearing age who were assigned to case or control groups according to their states of urogenital tract infections. The participants were also grouped by childbearing history and menstrual cycle. Vaginal swabs were collected and stored at - 70 °C until assayed. The V3-V4 region of 16S rRNA gene was amplified using PCR and sequenced on the Illumina MiSeq platform. We tested the hypothesis of whether the relative abundance of microbial species in vaginal microbiota was varied with urogenital tract infections, childbearing history and menstrual cycle. The vaginal microbial richness (Alpha diversity measured by PD_whole tree) was decreased in normal women (without reproductive tract infections) than in those with bacterial vaginosis (BV), and decreased in pregnant women than in other groups of non-pregnancy. Similarly, women from groups of normal and in pregnancy had lower beta diversity on measure of unweighted_unifrac distance in comparison to those of infected and non-pregnant. The top 10 genus relative abundance, especially Lactobacillus, which was the most dominant genus with the relative abundance of 71.55% among all samples, did not differ significantly between groups of childbearing history and menstrual cycle analyzed by ANOVA and nonparametric kruskal_wallis. Lactobacillus iners and Lactobacillus helveticus have the most abundance, totally account for 97.92% relative abundance of genus Lactobacillus. We also found that a higher L.helveticus/L.iners ratio is more likely to present in normal women than in the infected and in pregnant than in non-pregnant, although these comparisons lack statistical significance.ConclusionsThe relative abundance of dominant bacterial taxa in vaginal microbial communities of women at childbearing age were not different among groups of childbearing history and menstrual cycle. Women from groups of in pregnancy and without reproductive tract infections had lower alpha and beta diversity. The composition of the main lactobacillus species may shift upon phases of a menstrual cycle and the status of reproductive tract infections.

Dataset Information

A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle.

Publications

A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle.

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