Dataset Information

Phase-transitional Fe₃O₄/perfluorohexane Microspheres for Magnetic Droplet Vaporization.

ABSTRACT: Activating droplets vaporization has become an attractive strategy for ultrasound imaging and physical therapy due to the significant increase in ultrasound backscatter signals and its ability to physically damage the tumor cells. However, the current two types of transitional droplets named after their activation methods have their respective limitations. To circumvent the limitations of these activation methods, here we report the concept of magnetic droplet vaporization (MDV) for stimuli-responsive cancer theranostics by a magnetic-responsive phase-transitional agent. This magnetic-sensitive phase-transitional agent-perfluorohexane (PFH)-loaded porous magnetic microspheres (PFH-PMMs), with high magnetic-thermal energy-transfer capability, could quickly respond to external alternating current (AC) magnetic fields to produce thermal energy and trigger the vaporization of the liquid PFH. We systematically demonstrated MDV both in vitro and in vivo. This novel trigger method with deep penetration can penetrate the air-filled viscera and trigger the vaporization of the phase-transitional agent without the need of pre-focusing lesion. This unique MDV strategy is expected to substantially broaden the biomedical applications of nanotechnology and promote the clinical treatment of tumors that are not responsive to chemical therapies.

SUBMITTER: Wang R

PROVIDER: S-EPMC5381248 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Phase-transitional Fe<sub>3</sub>O<sub>4</sub>/perfluorohexane Microspheres for Magnetic Droplet Vaporization.

Wang Ronghui R Zhou Yang Y Zhang Ping P Chen Yu Y Gao Wei W Xu Jinshun J Chen Hangrong H Cai Xiaojun X Zhang Kun K Li Pan P Wang Zhigang Z Hu Bing B Ying Tao T Zheng Yuanyi Y

Theranostics 20170208 4

Activating droplets vaporization has become an attractive strategy for ultrasound imaging and physical therapy due to the significant increase in ultrasound backscatter signals and its ability to physically damage the tumor cells. However, the current two types of transitional droplets named after their activation methods have their respective limitations. To circumvent the limitations of these activation methods, here we report the concept of magnetic droplet vaporization (MDV) for stimuli-resp ...[more]

PMID: 28382158

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Project description:One of the major challenges in developing acoustic droplet vaporization (ADV)-associated therapy as an effective and safe strategy is the precise determination of the spatial cellular bioeffects after ADV (cell death or cell membrane permeabilization). In this study, we combined high-speed camera imaging and live-cell microscopic imaging to observe the transient dynamics of droplets during ADV and to evaluate the mechanical force on cells. Methods: C6 glioma cells were co-incubated with DiI-labeled droplets (radius: 1.5, 2.25, and 3.0 μm). We used an acousto-optical system for high-speed bright-field (500 kfps) and fluorescence (40 kfps) microscopic imaging in order to visualize the dynamics of droplets under ultrasound excitation (frequency = 5 MHz, pressure = 5-8 MPa, cycle number = 3, pulse number = 1). Live-cell microscopic imaging was used to monitor the cell morphology, cell membrane permeabilization, and cell viability by membrane-anchored Lyn-yellow fluorescence protein, propidium Iodide staining, and calcein blue AM staining, respectively. Results: We discovered that the spatial distribution of ADV-induced bioeffects could be mapped to the physical dynamics of droplet vaporization. For droplets with a 1.5 μm radius, the distance threshold for ADV-induced cell death (5.5±1.9 μm) and reversible membrane permeabilization (11.3±3.5 μm) was well correlated with the distance of ADV-bubble pressing downward to the floor (5.7±1.3 μm) and maximum distance of droplet expansion (11.5±2.6 μm), respectively. These distances were enlarged by increasing the droplet sizes and insonation acoustic pressures. The live-cell imaging results show that ADV-bubbles can directly disrupt the cell membrane layer and induce intensive intracellular substance leakage. Further, the droplets shed the payload onto nearby cells during ADV, suggesting ADV could directly induce adjacent cell death by physical force and enhancement of chemotherapy to distant cells. Conclusion: This study provide new insights into the ADV-mediated physicochemical synergic effect for medical applications.

Dataset Information

Phase-transitional Fe₃O₄/perfluorohexane Microspheres for Magnetic Droplet Vaporization.

Publications

Phase-transitional Fe<sub>3</sub>O<sub>4</sub>/perfluorohexane Microspheres for Magnetic Droplet Vaporization.

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Dataset Information

Phase-transitional Fe3O4/perfluorohexane Microspheres for Magnetic Droplet Vaporization.

Publications

Phase-transitional Fe<sub>3</sub>O<sub>4</sub>/perfluorohexane Microspheres for Magnetic Droplet Vaporization.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

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Phase-transitional Fe₃O₄/perfluorohexane Microspheres for Magnetic Droplet Vaporization.