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Crowding-induced cooperativity in DNA surface hybridization.

ABSTRACT: High density DNA brush is not only used to model cellular crowding, but also has a wide application in DNA-functionalized materials. Experiments have shown complicated cooperative hybridization/melting phenomena in these systems, raising the question that how molecular crowding influences DNA hybridization. In this work, a theoretical modeling including all possible inter and intramolecular interactions, as well as molecular details for different species, is proposed. We find that molecular crowding can lead to two distinct cooperative behaviours: negatively cooperative hybridization marked by a broader transition width, and positively cooperative hybridization with a sharper transition, well reconciling the experimental findings. Moreover, a phase transition as a result of positive cooperativity is also found. Our study provides new insights in crowding and compartmentation in cell, and has the potential value in controlling surface morphologies of DNA functionalized nano-particles.

SUBMITTER: Lei QL

PROVIDER: S-EPMC5381746 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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Crowding-induced cooperativity in DNA surface hybridization.

Lei Qun-li QL Ren Chun-lai CL Su Xiao-hang XH Ma Yu-qiang YQ

Scientific reports 20150415

High density DNA brush is not only used to model cellular crowding, but also has a wide application in DNA-functionalized materials. Experiments have shown complicated cooperative hybridization/melting phenomena in these systems, raising the question that how molecular crowding influences DNA hybridization. In this work, a theoretical modeling including all possible inter and intramolecular interactions, as well as molecular details for different species, is proposed. We find that molecular crow ...[more]

PMID: 25875056

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