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Expansion of FasL-Expressing CD5⁺ B Cells in Type 1 Diabetes Patients.

ABSTRACT: Fas ligand drives insulitis in the non-obese diabetic mouse model of type 1 diabetes (T1D) and negatively regulates IL-10-producing (IL-10^pos) CD5⁺ B cells in pancreata. Relevance of these phenomena to the human disease is poorly understood. Here, using splenocytes from T1D, autoantibody (Ab⁺), and non-diabetic (ND) human subjects, we show that a subpopulation of CD5⁺ B cells that is characterized by expression of FasL (FasL^hiCD5⁺) was significantly elevated in T1D subjects, many of whom had significantly reduced frequency of IL-10^posCD5⁺ B cells compared to Ab⁺ subjects. The majority of FasL^hiCD5⁺ B cells did not produce cytokines and were more highly resistant to activation-induced cell death than their IL-10^posCD5⁺ counterparts. These results associate expansion of FasL-expressing CD5⁺ B cells with T1D and lay the groundwork for future mechanistic studies to understand specific role in disease pathogenesis.

SUBMITTER: Saxena A

PROVIDER: S-EPMC5383713 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Expansion of FasL-Expressing CD5+ B Cells in Type 1 Diabetes Patients.

Saxena Ankit A Yagita Hideo H Donner Thomas W TW Hamad Abdel Rahim A ARA

Frontiers in immunology 20170407

Fas ligand drives insulitis in the non-obese diabetic mouse model of type 1 diabetes (T1D) and negatively regulates IL-10-producing (IL-10pos) CD5+ B cells in pancreata. Relevance of these phenomena to the human disease is poorly understood. Here, using splenocytes from T1D, autoantibody (Ab+), and non-diabetic (ND) human subjects, we show that a subpopulation of CD5+ B cells that is characterized by expression of FasL (FasLhiCD5+ ...[more]

PMID: 28439273

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