Browse
Submit Data
Databases
API
Help

Dataset Information

26 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome Sequence of a Toxin-Positive Clostridium difficile Strain Isolated from Murine Feces.

ABSTRACT: Herein, we report the genome sequence of a Clostridium difficile strain isolated from the feces of antibiotic-treated C57BL/6 mice. We have named this strain, which differs considerably from those of the previously sequenced C. difficile strains, LEM1.

SUBMITTER: Etienne-Mesmin L

PROVIDER: S-EPMC5383883 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Genome Sequence of a Toxin-Positive Clostridium difficile Strain Isolated from Murine Feces.

Etienne-Mesmin Lucie L Chassaing Benoit B Adekunle Oluwaseyi O Mattei Lisa M LM Edwards Adrianne N AN McBride Shonna M SM Bushman Frederic D FD Gewirtz Andrew T AT

Genome announcements 20170406 14

Herein, we report the genome sequence of a Clostridium difficile strain isolated from the feces of antibiotic-treated C57BL/6 mice. We have named this strain, which differs considerably from those of the previously sequenced C. difficile strains, LEM1. ...[more]

PMID: 28385835

Similar Datasets

Draft Genome Sequence of Clostridium butyricum Strain 16-3, Isolated from Neonatal Feces.

Project description:Here, we report the genome sequence of Clostridium butyricum strain 16-3, which was isolated from infant feces. The genome contains circular contigs of 3,861,515 bp and 769,300 bp, with G+C contents of 28.8% and 28.3%, respectively.

| S-EPMC9387228 | biostudies-literature

Draft Genome Sequence of Clostridium sp. Strain chh4-2 Isolated from Human Feces.

Project description:Here, we report the draft genome sequence of a Clostridium sp. strain isolated from a fecal sample of a 34-year-old adult male in Taiwan. This strain may represent a new bacterium, as suggested by a comparison based on whole-genome sequencing. The genome assembly comprised 6,089,737 bp, with a 45.63% G+C content.

| S-EPMC5824015 | biostudies-literature

Complete Genome Sequence of Clostridium butyricum Strain DKU_butyricum 4-1, Isolated from Infant Feces.

Project description:Clostridium butyricum is a strictly anaerobic spore-forming bacillus that is commonly present in the gut of humans. We report here the complete genome sequence of Clostridium butyricum strain DKU_butyricum 4-1, isolated from infant feces.

| S-EPMC7171215 | biostudies-literature

Genome Sequence of a Clostridioides difficile Strain Isolated from Feces from a Patient in Southern Thailand.

Project description:Clostridioides difficile is a Gram-positive, obligate anaerobic, toxin-producing bacillus that is linked to antibiotic-associated diarrhea. Here, we report the whole-genome sequence of a C. difficile strain isolated from stool from a patient, using next-generation sequencing (MGISEG-2000). De novo assembly revealed a genome length of 4,208,266 bp. Multilocus sequence typing (MLST) results showed that the isolate belonged to sequence type 23 (ST23).

| S-EPMC10353394 | biostudies-literature

Characterization of a toxin A-negative, toxin B-positive strain of Clostridium difficile responsible for a nosocomial outbreak of Clostridium difficile-associated diarrhea.

Project description:Clostridium difficile-associated diarrhea (CAD) is a very common nosocomial infection that contributes significantly to patient morbidity and mortality as well as to the cost of hospitalization. Previously, strains of toxin A-negative, toxin B-positive C. difficile were not thought to be associated with clinically significant disease. This study reports the characterization of a toxin A-negative, toxin B-positive strain of C. difficile that was responsible for a recently described nosocomial outbreak of CAD. Analysis of the seven patient isolates from the outbreak by pulsed-field gel electrophoresis indicated that this outbreak was due to transmission of a single strain of C. difficile. Our characterization of this strain (HSC98) has demonstrated that the toxin A gene lacks 1.8 kb from the carboxy repetitive oligopeptide (CROP) region but apparently has no other major deletions from other regions of the toxin A or toxin B gene. The remaining 1.3-kb fragment of the toxin A CROP region from strain HSC98 showed 98% sequence homology with strain 1470, previously reported by M. Weidmann in 1997 (GenBank accession number Y12616), suggesting that HSC98 is toxinotype VIII. The HSC98 strain infecting patients involved in this outbreak produced the full spectrum of clinical illness usually associated with C. difficile-associated disease. This pathogenic spectrum was manifest despite the inability of this strain to alter tight junctions as determined by using in vitro tissue culture testing, which suggested that no functional toxin A was produced by this strain.

| S-EPMC87004 | biostudies-literature

Complete genome sequence of a Clostridioides difficile cryptic C-III strain isolated from horse feces.

Project description:We provide the complete genome of a non-toxigenic Clostridioides difficile strain isolated from horse feces. The strain represents a sub-cluster in the cryptic clade C-III. The genome consists of one chromosome (4,144,784 bp) and one plasmid (10,144 bp) and encodes 3,798 putative genes.

| S-EPMC10720495 | biostudies-literature

Draft genome sequence of the nontoxigenic Clostridium difficile strain CD37.

Project description:Here we report the draft genome sequence of Clostridium difficile strain CD37, the first nontoxigenic strain sequenced. Every sequenced strain of Clostridium difficile has been shown to contain multiple different mobile genetic elements. The draft genome sequence of strain CD37 reveals the presence of two putative conjugative transposons.

| S-EPMC3318468 | biostudies-literature

Complete Genome Sequence of Peptacetobacter (Clostridium) hiranonis Strain DGF055142, Isolated from Dog Feces from Flagstaff, Arizona, USA, 2019.

Project description:A single-chromosome closed genome of Peptacetobacter (Clostridium) hiranonis strain DGF055142 was generated using Illumina MiSeq short reads paired with Oxford Nanopore MinION long reads. This isolate was obtained from a canine in Flagstaff, Arizona, in 2019. Peptacetobacter (C.) hiranonis was hypothesized to contribute to canine Clostridium difficile infection resistance.

| S-EPMC7936630 | biostudies-literature

Complete Genome Sequence of the Clostridium difficile Type Strain DSM 1296T.

Project description:In this study, we sequenced the complete genome of the Clostridium difficile type strain DSM 1296(T). A combination of single-molecule real-time (SMRT) and Illumina sequencing technology revealed the presence of one chromosome and two extrachromosomal elements, the bacteriophage phiCDIF1296T and a putative plasmid-like structure harboring genes of another bacteriophage.

| S-EPMC4599105 | biostudies-literature

A new type of toxin A-negative, toxin B-positive Clostridium difficile strain lacking a complete tcdA gene.

Project description:Toxins A and B are the main virulence factors of Clostridium difficile and are the targets for molecular diagnostic tests. Here, we describe a new toxin A-negative, toxin B-positive, binary toxin CDT (Clostridium difficile transferase)-negative (A(-) B(+) CDT(-)) toxinotype (XXXII) characterized by a variant type of pathogenicity locus (PaLoc) without tcdA and with atypical organization of the PaLoc integration site.

| S-EPMC4298534 | biostudies-literature