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Global Post-Translational Modification Discovery.

ABSTRACT: A new global post-translational modification (PTM) discovery strategy, G-PTM-D, is described. A proteomics database containing UniProt-curated PTM information is supplemented with potential new modification types and sites discovered from a first-round search of mass spectrometry data with ultrawide precursor mass tolerance. A second-round search employing the supplemented database conducted with standard narrow mass tolerances yields deep coverage and a rich variety of peptide modifications with high confidence in complex unenriched samples. The G-PTM-D strategy represents a major advance to the previously reported G-PTM strategy and provides a powerful new capability to the proteomics research community.

SUBMITTER: Li Q

PROVIDER: S-EPMC5387672 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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Global Post-Translational Modification Discovery.

Li Qiyao Q Shortreed Michael R MR Wenger Craig D CD Frey Brian L BL Schaffer Leah V LV Scalf Mark M Smith Lloyd M LM

Journal of proteome research 20170301 4

A new global post-translational modification (PTM) discovery strategy, G-PTM-D, is described. A proteomics database containing UniProt-curated PTM information is supplemented with potential new modification types and sites discovered from a first-round search of mass spectrometry data with ultrawide precursor mass tolerance. A second-round search employing the supplemented database conducted with standard narrow mass tolerances yields deep coverage and a rich variety of peptide modifications wit ...[more]

PMID: 28248113

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