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Antimicrobial photodynamic activity and cytocompatibility of Au₂₅(Capt)₁₈ clusters photoexcited by blue LED light irradiation.

ABSTRACT: Antimicrobial photodynamic therapy (aPDT) has beneficial effects in dental treatment. We applied captopril-protected gold (Au₂₅(Capt)₁₈) clusters as a novel photosensitizer for aPDT. Photoexcited Au clusters under light irradiation generated singlet oxygen (¹O₂). Accordingly, the antimicrobial and cytotoxic effects of Au₂₅(Capt)₁₈ clusters under dental blue light-emitting diode (LED) irradiation were evaluated. ¹O₂ generation of Au₂₅(Capt)₁₈ clusters under blue LED irradiation (420-460 nm) was detected by a methotrexate (MTX) probe. The antimicrobial effects of photoexcited Au clusters (0, 5, 50, and 500 ?g/mL) on oral bacterial cells, such as Streptococcus mutans, Aggregatibacter actinomycetemcomitans, and Porphyromonas gingivalis, were assessed by morphological observations and bacterial growth experiments. Cytotoxicity testing of Au clusters and blue LED irradiation was then performed against NIH3T3 and MC3T3-E1 cells. In addition, the biological performance of Au clusters (500 ?g/mL) was compared to an organic dye photosensitizer, methylene blue (MB; 10 and 100 ?g/mL). We confirmed the ¹O₂ generation ability of Au₂₅(Capt)₁₈ clusters through the fluorescence spectra of oxidized MTX. Successful application of photoexcited Au clusters to aPDT was demonstrated by dose-dependent decreases in the turbidity of oral bacterial cells. Morphological observation revealed that application of Au clusters stimulated destruction of bacterial cell walls and inhibited biofilm formation. Aggregation of Au clusters around bacterial cells was fluorescently observed. However, photoexcited Au clusters did not negatively affect the adhesion, spreading, and proliferation of mammalian cells, particularly at lower doses. In addition, application of Au clusters demonstrated significantly better cytocompatibility compared to MB. We found that a combination of Au₂₅(Capt)₁₈ clusters and blue LED irradiation exhibited good antimicrobial effects through ¹O₂ generation and biosafe characteristics, which is desirable for aPDT in dentistry.

SUBMITTER: Miyata S

PROVIDER: S-EPMC5388257 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Antimicrobial photodynamic activity and cytocompatibility of Au25(Capt)18 clusters photoexcited by blue LED light irradiation.

Miyata Saori S Miyaji Hirofumi H Kawasaki Hideya H Yamamoto Masaki M Nishida Erika E Takita Hiroko H Akasaka Tsukasa T Ushijima Natsumi N Iwanaga Toshihiko T Sugaya Tsutomu T

International journal of nanomedicine 20170404

Antimicrobial photodynamic therapy (aPDT) has beneficial effects in dental treatment. We applied captopril-protected gold (Au25(Capt)18) clusters as a novel photosensitizer for aPDT. Photoexcited Au clusters under light irradiation generated singlet oxygen (1O2). Accordingly, the antimicrobial and cytotoxic effects of Au25(Capt)18 clusters under dental blue light-emitting diode (LED) irradiation were evaluated. 1O2 ...[more]

PMID: 28435253

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Project description:The aggregates of microtubule-associated protein Tau are considered as a major hallmark of Alzheimer's disease. Tau aggregates accumulate intracellularly leading to neuronal toxicity. Numerous approaches have been targeted against Tau protein aggregation, which include application of synthetic and natural compounds. Toluidine blue is a basic dye of phenothiazine family, which on irradiation with a 630 nm light gets converted into a photoexcited form, leading to generation of singlet oxygen species. Methylene blue is the parent compound of toluidine blue, which has been reported to be potent against tauopathy. In the present work, we studied the potency of toluidine blue and photoexcited toluidine blue against Tau aggregation. Biochemical and biophysical analyses using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, ThS fluorescence, circular dichroism spectroscopy, and electron microscopy suggested that toluidine blue inhibited the aggregation of Tau in vitro. The photoexcited toluidine blue potentially dissolved the matured Tau fibrils, which indicated the disaggregation property of toluidine blue. The cell biology studies including the cytotoxicity assay and reactive oxygen species (ROS) production assay suggested toluidine blue to be a biocompatible dye as it reduced ROS levels and cell death. The photoexcited toluidine blue modulates the cytoskeleton network in cells, which was supported by immunofluorescence studies of neuronal cells. The studies in a UAS Tau E14 transgenic Drosophila model suggested that photoexcited toluidine blue was potent to restore the survival and memory deficits of Drosophila. The overall finding of our studies suggested toluidine blue to be a potent molecule in rescuing the Tau-mediated pathology by inhibiting its aggregation, reducing the cell death, and modulating the tubulin levels and behavioral characteristics of Drosophila. Thus, toluidine blue can be addressed as a potent molecule against Alzheimer's disease.

Dataset Information

Antimicrobial photodynamic activity and cytocompatibility of Au₂₅(Capt)₁₈ clusters photoexcited by blue LED light irradiation.

Publications

Antimicrobial photodynamic activity and cytocompatibility of Au<sub>25</sub>(Capt)<sub>18</sub> clusters photoexcited by blue LED light irradiation.

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