Ontology highlight
ABSTRACT:
SUBMITTER: Miyata N
PROVIDER: S-EPMC5392686 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Miyata Non N Tang Zhiye Z Conti Michael A MA Johnson Meghan E ME Douglas Colin J CJ Hasson Samuel A SA Damoiseaux Robert R Chang Chia-En A CA Koehler Carla M CM
The Journal of biological chemistry 20170206 13
Diverse protein import pathways into mitochondria use translocons on the outer membrane (TOM) and inner membrane (TIM). We adapted a genetic screen, based on Ura3 mistargeting from mitochondria to the cytosol, to identify small molecules that attenuated protein import. Small molecule mitochondrial import blockers of the Carla Koehler laboratory (MB)-10 inhibited import of substrates that require the TIM23 translocon. Mutational analysis coupled with molecular docking and molecular dynamics model ...[more]