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An Exploration of Traditional Chinese Medicinal Plants with Anti-Inflammatory Activities.

ABSTRACT: In a continuing effort to discover more anti-inflammatory medicinal plants in China, the anti-inflammatory activities of 101 extracts from different parts of 84 traditional medicinal plants were evaluated by a panel of in vitro and in vivo assays. Nuclear factor-kappa B (NF-?B) inhibitory effects were determined by luciferase assay in stably transfected Hela cells. Cytotoxic activities were assessed using the MTT assay. Inhibitory effects on LPS-induced nitric oxide production and proinflammatory mediators were assessed by Griess reaction and Real-Time PCR analysis, respectively. In vivo anti-inflammatory activities were examined by xylene-induced mice ear edema model. In total, 22 extracts showed promising NF-?B inhibitory effects whereas 9 of them did not affect the cell viability. The 9 hit extracts were active in at least one of the subsequently performed in vitro pharmacological test systems. The extract from Hemerocallis minor (root) was selected to perform the in vivo study because it demonstrated significant suppressive effects in all the in vitro assays. Results showed that the extract of Hemerocallis minor (Root) was able to alleviate ear edema effectively in xylene-induced mice ear edema mode. Collectively, our study provides evidence for the potential anti-inflammatory effects of the medicinal plants traditionally used in China. Further phytochemical and pharmacological studies remain to be clarified.

SUBMITTER: Fan C

PROVIDER: S-EPMC5394394 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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An Exploration of Traditional Chinese Medicinal Plants with Anti-Inflammatory Activities.

Fan Chao C Jin Hui-Zi HZ Wu Lehao L Zhang Yu Y Ye Richard D RD Zhang Weidong W Zhang Yan Y

Evidence-based complementary and alternative medicine : eCAM 20170404

In a continuing effort to discover more anti-inflammatory medicinal plants in China, the anti-inflammatory activities of 101 extracts from different parts of 84 traditional medicinal plants were evaluated by a panel of in vitro and in vivo assays. Nuclear factor-kappa B (NF-<i>κ</i>B) inhibitory effects were determined by luciferase assay in stably transfected Hela cells. Cytotoxic activities were assessed using the MTT assay. Inhibitory effects on LPS-induced nitric oxide production and proinfl ...[more]

PMID: 28473862

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An Exploration of Traditional Chinese Medicinal Plants with Anti-Inflammatory Activities.

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An Exploration of Traditional Chinese Medicinal Plants with Anti-Inflammatory Activities.

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