Ontology highlight
ABSTRACT:
SUBMITTER: Chen J
PROVIDER: S-EPMC5397178 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Chen Jian J Cai Tanxi T Zheng Chunwei C Lin Xiwen X Wang Guojun G Liao Shangying S Wang Xiuxia X Gan Haiyun H Zhang Daoqin D Hu Xiangjing X Wang Si S Li Zhen Z Feng Yanmin Y Yang Fuquan F Han Chunsheng C
Nucleic acids research 20170401 7
miRNAs play important roles during mammalian spermatogenesis. However, the function of most miRNAs in spermatogenesis and the underlying mechanisms remain unknown. Here, we report that miR-202 is highly expressed in mouse spermatogonial stem cells (SSCs), and is oppositely regulated by Glial cell-Derived Neurotrophic Factor (GDNF) and retinoic acid (RA), two key factors for SSC self-renewal and differentiation. We used inducible CRISPR-Cas9 to knockout miR-202 in cultured SSCs, and found that th ...[more]