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ABSTRACT: Purpose
To assess reproducibility of glutamate measurement in the human brain by two short echo time (TE) 1H-MRS sequences [stimulated echo acquisition mode (STEAM) and semi-localized by adiabatic selective refocusing (sLASER)] at 7 T. Reliable assessment of glutamate is important when studying a variety of neurological and neuropsychiatric disorders. At 7 T, the glutamate signal can be separated from the glutamine signal and hence more accurately measured as compared to lower field strengths. A sLASER sequence has been developed for 7 T, using field focusing at short TE, resulting in twice as much signal as can be obtained using STEAM and improved localization accuracy due to a decreased chemical shift artifact.Materials and methods
Eight subjects were scanned twice using both STEAM and sLASER. Data were acquired from the frontal and occipital brain region. Subsequently, intraclass correlations were computed for the estimated metabolite concentrations.Results
sLASER has higher ICC's for glutamate concentration as compared to STEAM in both the frontal and occipital VOI, which is probably due to the higher sensitivity and localization accuracy.Conclusion
We conclude that sLASER 1H-MRS at 7 T is a reliable method to obtain reproducible measures of glutamate levels in the human brain at such high accuracy that individual variability, even between age-matched subjects, is measured.
SUBMITTER: Marsman A
PROVIDER: S-EPMC5399075 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Marsman Anouk A Boer Vincent O VO Luijten Peter R PR Hulshoff Pol Hilleke E HE Klomp Dennis W J DWJ Mandl René C W RCW
Frontiers in psychiatry 20170421
<h4>Purpose</h4>To assess reproducibility of glutamate measurement in the human brain by two short echo time (TE) <sup>1</sup>H-MRS sequences [stimulated echo acquisition mode (STEAM) and semi-localized by adiabatic selective refocusing (sLASER)] at 7 T. Reliable assessment of glutamate is important when studying a variety of neurological and neuropsychiatric disorders. At 7 T, the glutamate signal can be separated from the glutamine signal and hence more accurately measured as compared to lower ...[more]