Dataset Information

Tissue accumulation of microplastics in mice and biomarker responses suggest widespread health risks of exposure.

ABSTRACT: Microplastics (MPs) are a significant environmental health issue and increasingly greater source of concern. MPs have been detected in oceans, rivers, sediments, sewages, soil and even table salts. MPs exposure on marine organisms and humans has been documented, but information about the toxicity of MPs in mammal is limited. Here we used fluorescent and pristine polystyrene microplastics (PS-MPs) particles with two diameters (5??m and 20??m) to investigate the tissue distribution, accumulation, and tissue-specific health risk of MPs in mice. Results indicated that MPs accumulated in liver, kidney and gut, with a tissue-accumulation kinetics and distribution pattern that was strongly depended on the MPs particle size. In addition, analyses of multiple biochemical biomarkers and metabolomic profiles suggested that MPs exposure induced disturbance of energy and lipid metabolism as well as oxidative stress. Interestingly, blood biomarkers of neurotoxicity were also altered. Our results uncovered the distribution and accumulation of MPs across mice tissues and revealed significant alteration in several biomarkers that indicate potential toxicity from MPs exposure. Collectively, our data provided new evidence for the adverse consequences of MPs.

SUBMITTER: Deng Y

PROVIDER: S-EPMC5402289 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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Tissue accumulation of microplastics in mice and biomarker responses suggest widespread health risks of exposure.

Deng Yongfeng Y Zhang Yan Y Lemos Bernardo B Ren Hongqiang H

Scientific reports 20170424

Microplastics (MPs) are a significant environmental health issue and increasingly greater source of concern. MPs have been detected in oceans, rivers, sediments, sewages, soil and even table salts. MPs exposure on marine organisms and humans has been documented, but information about the toxicity of MPs in mammal is limited. Here we used fluorescent and pristine polystyrene microplastics (PS-MPs) particles with two diameters (5 μm and 20 μm) to investigate the tissue distribution, accumulation, ...[more]

PMID: 28436478

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Project description:Microplastics (MPs)' ingestion has been demonstrated in several aquatic organisms. This process may facilitate the hydrophobic waterborne pollutants or chemical additives transfer to biota. In the present study the suitability of a battery of biomarkers on oxidative stress, physiology, tissue function and metabolic profile was investigated for the early detection of adverse effects of 21-day exposure to polystyrene microplastics (PS-MPs, sized 5-12 μm) in the liver and gills of zebrafish Danio rerio and perch, Perca fluviatilis, both of which are freshwater fish species. An optical volume map representation of the zebrafish gill by Raman spectroscopy depicted 5 μm diameter PS-MP dispersed in the gill tissue. Concentrations of PS-MPs close to the EC50 of each fish affected fish physiology in all tissues studied. Increased levels of biomarkers of oxidative damage in exposed fish in relation to controls were observed, as well as activation of apoptosis and autophagy processes. Malondialdehyde (MDA), protein carbonyls and DNA damage responses differed with regard to the sensitivity of each tissue of each fish. In the toxicity cascade gills seemed to be more liable to respond to PS-MPs than liver for the majority of the parameters measured. DNA damage was the most susceptible biomarker exhibiting greater response in the liver of both species. The interaction between MPs and cellular components provoked metabolic alterations in the tissues studied, affecting mainly amino acids, nitrogen and energy metabolism. Toxicity was species and tissue specific, with specific biomarkers responding differently in gills and in liver. The fish species that seemed to be more susceptible to MPs at the conditions studied, was P. fluviatilis compared to D. rerio. The current findings add to a holistic approach for the identification of small sized PS-MPs' biological effects in fish, thus aiming to provide evidence regarding PS-MPs' environmental impact on wild fish populations and food safety and adequacy.

Project description:Earthworms (Eisenia fetida) were exposed to OECD soils contaminated with arsenite (29.3 mg kg-1), arsenate (35.2 mg kg-1), monomethylarsonate (342.5 mg kg-1) and dimethylarsinate (373.0 mg kg-1) for 64 days. The exposure concentration for the four arsenic species was set at one-tenth of 14 d-LC50 in order to compare their toxicity. Eight biomarkers including superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, glutathione reductase, reduced glutathione, lipid peroxidation and metallothioneins were analyzed in the organisms. A multi-biomarker approach, the integrated biomarker response (IBR) index, was adopted to summarize the multi-biomarker responses to a single value, reflecting the integrated stress of different arsenic species on earthworms. Furthermore, total arsenic and arsenic speciation were analyzed in earthworm tissue to evaluate the relationship between arsenic accumulation and biomarker responses at the molecular and subcellular levels and to observe the role of arsenic biotransformation in earthworms. The results showed that the toxicity of the four arsenic species was ranked as: arsenite > arsenate > monomethylarsonate and dimethylarsinate. Although organic arsenics showed a low degree of biotoxicity, they could be turned into highly toxic inorganic arsenics under the effect of demethylation, which caused a toxic effect on organisms. The biomarker responses indicated that a sub-lethal dose of both arsenite and arsenate could trigger the response of the antioxidant defense system and cause oxidative damage when the protective capacity of the system was exhausted. Arsenic in earthworms could be detoxified during the process of biotransformation, where inorganic arsenics were converted into organic arsenics, which would then be excreted out. Based on these results, it was proved that different arsenic species showed different degrees of toxicity. Therefore, arsenic species should be differentiated in order to obtain accurate results in quality/risk assessment programs.

Dataset Information

Tissue accumulation of microplastics in mice and biomarker responses suggest widespread health risks of exposure.

Publications

Tissue accumulation of microplastics in mice and biomarker responses suggest widespread health risks of exposure.

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