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Accurate identification of single-nucleotide variants in whole-genome-amplified single cells.

ABSTRACT: Mutation analysis in single-cell genomes is prone to artifacts associated with cell lysis and whole-genome amplification. Here we addressed these issues by developing single-cell multiple displacement amplification (SCMDA) and a general-purpose single-cell-variant caller, SCcaller (https://github.com/biosinodx/SCcaller/). By comparing SCMDA-amplified single cells with unamplified clones from the same population, we validated the procedure as a firm foundation for standardized somatic-mutation analysis in single-cell genomics.

SUBMITTER: Dong X

PROVIDER: S-EPMC5408311 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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Accurate identification of single-nucleotide variants in whole-genome-amplified single cells.

Dong Xiao X Zhang Lei L Milholland Brandon B Lee Moonsook M Maslov Alexander Y AY Wang Tao T Vijg Jan J

Nature methods 20170320 5

Mutation analysis in single-cell genomes is prone to artifacts associated with cell lysis and whole-genome amplification. Here we addressed these issues by developing single-cell multiple displacement amplification (SCMDA) and a general-purpose single-cell-variant caller, SCcaller (https://github.com/biosinodx/SCcaller/). By comparing SCMDA-amplified single cells with unamplified clones from the same population, we validated the procedure as a firm foundation for standardized somatic-mutation an ...[more]

PMID: 28319112

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