Uncoupling neurogenic gene networks in the Drosophila embryo.
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ABSTRACT:
SUBMITTER: Rogers WA
PROVIDER: S-EPMC5411704 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Publications
Uncoupling neurogenic gene networks in the <i>Drosophila</i> embryo.
Rogers William A WA Goyal Yogesh Y Yamaya Kei K Shvartsman Stanislav Y SY Levine Michael S MS
Genes & development 20170420 7
The EGF signaling pathway specifies neuronal identities in the <i>Drosophila</i> embryo by regulating developmental patterning genes such as <i>intermediate neuroblasts defective</i> (<i>ind</i>). EGFR is activated in the ventral midline and neurogenic ectoderm by the Spitz ligand, which is processed by the Rhomboid protease. CRISPR/Cas9 was used to delete defined <i>rhomboid</i> enhancers mediating expression at each site of Spitz processing. Surprisingly, the neurogenic ectoderm, not the ventr ...[more]
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