Ontology highlight
ABSTRACT:
SUBMITTER: Sinha S
PROVIDER: S-EPMC5413072 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Sinha Supriya S Li Fuyang F Villarreal Diana D Shim Jae Hoon JH Yoon Suhyeon S Myung Kyungjae K Shim Eun Yong EY Lee Sang Eun SE
PLoS genetics 20170418 4
Microhomology (MH) flanking a DNA double-strand break (DSB) drives chromosomal rearrangements but its role in mutagenesis has not yet been analyzed. Here we determined the mutation frequency of a URA3 reporter gene placed at multiple locations distal to a DSB, which is flanked by different sizes (15-, 18-, or 203-bp) of direct repeat sequences for efficient repair in budding yeast. Induction of a DSB accumulates mutations in the reporter gene situated up to 14-kb distal to the 15-bp MH, but more ...[more]