15-Deoxy-Δ^12,14-prostaglandin J₂ Exerts Antioxidant Effects While Exacerbating Inflammation in Mice Subjected to Ureteral Obstruction.

ABSTRACT: Urinary obstruction is associated with inflammation and oxidative stress, leading to renal dysfunction. Previous studies have shown that 15-deoxy-Δ^12,14-prostaglandin J₂ (15d-PGJ₂) has both antioxidant and anti-inflammatory effects. Using a unilateral ureteral obstruction (UUO) mouse model, we examined the effects of 15d-PGJ₂ on oxidative stress and inflammation in the kidney. Mice were subjected to UUO for 3 days and treated with 15d-PGJ₂. Protein and RNA expression were examined using immunoblotting and qPCR. 15d-PGJ₂ increased NF-E2-related nuclear factor erythroid-2 (Nrf2) protein expression in response to UUO, and heme oxygenase 1 (HO-1), a downstream target of Nrf2, was induced by 15d-PGJ₂. Additionally, 15d

SUBMITTER: Nilsson L 

PROVIDER: S-EPMC5414590 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature