Ontology highlight
ABSTRACT:
SUBMITTER: Wang G
PROVIDER: S-EPMC5415875 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Wang Guanbo G de Jong Rob N RN van den Bremer Ewald T J ETJ Parren Paul W H I PWHI Heck Albert J R AJR
Analytical chemistry 20170413 9
The determination of molecular weights (MWs) of heavily glycosylated proteins is seriously hampered by the physicochemical characteristics and heterogeneity of the attached carbohydrates. Glycosylation impacts protein migration during sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and size-exclusion chromatography (SEC) analysis. Standard electrospray ionization (ESI)-mass spectrometry does not provide a direct solution as this approach is hindered by extensive interferen ...[more]