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Human CTF18-RFC clamp-loader complexed with non-synthesising DNA polymerase ? efficiently loads the PCNA sliding clamp.


ABSTRACT: The alternative proliferating-cell nuclear antigen (PCNA)-loader CTF18-RFC forms a stable complex with DNA polymerase ? (Pol?). We observed that, under near-physiological conditions, CTF18-RFC alone loaded PCNA inefficiently, but loaded it efficiently when complexed with Pol?. During efficient PCNA loading, CTF18-RFC and Pol? assembled at a 3? primer-template junction cooperatively, and directed PCNA to the loading site. Site-specific photo-crosslinking of directly interacting proteins at the primer-template junction showed similar cooperative binding, in which the catalytic N-terminal portion of Pol? acted as the major docking protein. In the PCNA-loading intermediate with ATP?S, binding of CTF18 to the DNA structures increased, suggesting transient access of CTF18-RFC to the primer terminus. Pol? placed in DNA synthesis mode using a substrate DNA with a deoxidised 3? primer end did not stimulate PCNA loading, suggesting that DNA synthesis and PCNA loading are mutually exclusive at the 3? primer-template junction. Furthermore, PCNA and CTF18-RFC-Pol? complex engaged in stable trimeric assembly on the template DNA and synthesised DNA efficiently. Thus, CTF18-RFC appears to be involved in leading-strand DNA synthesis through its interaction with Pol?, and can load PCNA onto DNA when Pol? is not in DNA synthesis mode to restore DNA synthesis.

SUBMITTER: Fujisawa R 

PROVIDER: S-EPMC5416766 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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Human CTF18-RFC clamp-loader complexed with non-synthesising DNA polymerase ε efficiently loads the PCNA sliding clamp.

Fujisawa Ryo R   Ohashi Eiji E   Hirota Kouji K   Tsurimoto Toshiki T  

Nucleic acids research 20170501 8


The alternative proliferating-cell nuclear antigen (PCNA)-loader CTF18-RFC forms a stable complex with DNA polymerase ε (Polε). We observed that, under near-physiological conditions, CTF18-RFC alone loaded PCNA inefficiently, but loaded it efficiently when complexed with Polε. During efficient PCNA loading, CTF18-RFC and Polε assembled at a 3΄ primer-template junction cooperatively, and directed PCNA to the loading site. Site-specific photo-crosslinking of directly interacting proteins at the pr  ...[more]

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