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Comparative Proteomic Analysis of Mycobacterium tuberculosis Lineage 7 and Lineage 4 Strains Reveals Differentially Abundant Proteins Linked to Slow Growth and Virulence.


ABSTRACT: In order to decipher the nature of the slowly growing Mycobacterium tuberculosis (M.tuberculosis) lineage 7, the differentially abundant proteins in strains of M. tuberculosis lineage 7 and lineage 4 were defined. Comparative proteomic analysis by mass spectrometry was employed to identify, quantitate and compare the protein profiles of strains from the two M. tuberculosis lineages. Label-free peptide quantification of whole cells from M. tuberculosis lineage 7 and 4 yielded the identification of 2825 and 2541 proteins, respectively. A combined total of 2867 protein groups covering 71% of the predicted M. tuberculosis proteome were identified. The abundance of 125 proteins in M. tuberculosis lineage 7 and 4 strains was significantly altered. Notably, the analysis showed that a number of M. tuberculosis proteins involved in growth and virulence were less abundant in lineage 7 strains compared to lineage 4. Five ABC transporter proteins, three phosphate binding proteins essential for inorganic phosphate uptake, and six components of the type 7 secretion system ESX-3 involved in iron acquisition were less abundant in M. tuberculosis lineage 7. This proteogenomic analysis provided an insight into the lineage 7-specific protein profile which may provide clues to understanding the differential properties of lineage 7 strains in terms of slow growth, survival fitness, and pathogenesis.

SUBMITTER: Yimer SA 

PROVIDER: S-EPMC5423352 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Comparative Proteomic Analysis of <i>Mycobacterium tuberculosis</i> Lineage 7 and Lineage 4 Strains Reveals Differentially Abundant Proteins Linked to Slow Growth and Virulence.

Yimer Solomon A SA   Birhanu Alemayehu G AG   Kalayou Shewit S   Riaz Tahira T   Zegeye Ephrem D ED   Beyene Getachew T GT   Holm-Hansen Carol C   Norheim Gunnstein G   Abebe Markos M   Aseffa Abraham A   Tønjum Tone T  

Frontiers in microbiology 20170509


In order to decipher the nature of the slowly growing <i>Mycobacterium tuberculosis (M</i>.tuberculosis) lineage 7, the differentially abundant proteins in strains of <i>M. tuberculosis</i> lineage 7 and lineage 4 were defined. Comparative proteomic analysis by mass spectrometry was employed to identify, quantitate and compare the protein profiles of strains from the two <i>M. tuberculosis</i> lineages. Label-free peptide quantification of whole cells from <i>M. tuberculosis</i> lineage 7 and 4  ...[more]

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