N_ε- and O-Acetylation in Mycobacterium tuberculosis Lineage 7 and Lineage 4 Strains: Proteins Involved in Bioenergetics, Virulence, and Antimicrobial Resistance Are Acetylated.

ABSTRACT: Increasing evidence demonstrates that lysine acetylation is involved in Mycobacterium tuberculosis (Mtb) virulence and pathogenesis. However, previous investigations in Mtb have only monitored acetylation at lysine residues using selected reference strains. We analyzed the global N_ε- and O-acetylation of three Mtb isolates: two lineage 7 clinical isolates and the lineage 4 H37Rv reference strain. Quantitative acetylome analysis resulted in identification of 2490 class-I acetylation sites, 2349 O-acetylation and 141 N_ε-acetylation sites, derived from 953 unique proteins. Mtb O-acetylation was thereby significantly more abundant than N_ε-acetylation. The acetylated proteins were found to be involved in central metabolism, translation, stress responses, and ant

SUBMITTER: Birhanu AG 

PROVIDER: S-EPMC5669810 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature