Ontology highlight
ABSTRACT:
SUBMITTER: Lopez-Lopez P
PROVIDER: S-EPMC5423754 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
López-López P P Martínez-López M C MC Boldo-León X M XM Hernández-Díaz Y Y González-Castro T B TB Tovilla-Zárate C A CA Luna-Arias J P JP
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 20170403 4
Amebiasis is one of the twenty major causes of disease in Mexico; however, the diagnosis is difficult due to limitations of conventional microscopy-based techniques. In this study, we analyzed stool samples using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to differentiate between Entamoeba histolytica (pathogenic) and E. dispar (non-pathogenic). The target for the PCR amplification was a small region (228 bp) of the adh112 gene selected to increase the sensitivi ...[more]