Ontology highlight
ABSTRACT:
SUBMITTER: Hamzah Z
PROVIDER: S-EPMC1594701 | biostudies-literature | 2006 Sep
REPOSITORIES: biostudies-literature
Hamzah Zulhainan Z Petmitr Songsak S Mungthin Mathirut M Leelayoova Saovanee S Chavalitshewinkoon-Petmitr Porntip P
Journal of clinical microbiology 20060901 9
A single-round PCR assay was developed for detection and differential diagnosis of the three Entamoeba species found in humans, Entamoeba moshkovskii, Entamoeba histolytica, and Entamoeba dispar, that are morphologically identical as both cysts and trophozoites. A conserved forward primer was derived from the middle of the small-subunit rRNA gene, and reverse primers were designed from signature sequences specific to each of these three Entamoeba species. PCR generates a 166-bp product with E. h ...[more]