Activity-based protein profiling as a robust method for enzyme identification and screening in extremophilic Archaea.

ABSTRACT: Archaea are characterized by a unique life style in often environmental extremes but their thorough investigation is currently hampered by a limited set of suitable in vivo research methodologies. Here, we demonstrate that in vivo activity-based protein profiling (ABPP) may be used to sensitively detect either native or heterogeneously expressed active enzymes in living archaea even under these extreme conditions. In combination with the development of a genetically engineered archaeal screening strain, ABPP can furthermore be used in functional enzyme screenings from (meta)genome samples. We anticipate that our ABPP approach may therefore find application in basic archaeal research but also in the discovery of novel enzymes from (meta)genome libraries.

SUBMITTER: Zweerink S 

PROVIDER: S-EPMC5424146 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature