Ontology highlight
ABSTRACT:
SUBMITTER: Ji F
PROVIDER: S-EPMC5425148 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Ji Fang F Mokoena Mduduzi P MP Zhao Hongyan H Olaniran Ademola O AO Shi Jianrong J
PloS one 20170510 5
A colloidal gold (ICS) test was developed for rapid detection of zearalenone (ZEN) in wheat samples. The mAb against ZEN was prepared in our laboratory and labelled with colloidal gold as a probe for the ICS test. The conditions were optimized and 30 nm colloidal gold nanoparticles were chosen for optimal performance. Millipore 135 was chosen as the NC membrane for its level of sensitivity. The optimum amount of coated antigen ZEN-OVA and anti-ZEN mAb was 0.5 mg/mL and 8 μg/mL, respectively. The ...[more]