Dataset Information

Mutations in Hepatitis-B X-Gene Region: Chronic Hepatitis-B versus Cirrhosis.

ABSTRACT:

Introduction

Specific mutations in Hepatitis-B Virus (HBV) genome would proceed the development of chronic hepatitis B to more serious consequences like cirrhosis and end-stage liver disease.

Aim

This study was designed to detect deletion and insertion mutational patterns in the X-gene region in a population of chronic HBV and related cirrhosis patients.

Materials and methods

Sixty eight chronic HBV patients and 34 HBV-related cirrhotics were recruited from the eligible cases (N=50) referred to the academic hospitals of Gorgan city, Northeast of Iran, between Jan 2011 to Dec 2013. The HBx region was amplified by semi-nested PCR using serum samples and analyzed by sequencing.

Results

Our findings showed deletions and insertions in the C-terminal of HBx of the cirrhotic group and 8 bp found in two chronic HBV cases (2.9%). We detected 15 types of deletions in cirrhotic cases such as 1762-1768, 1763-1770, 1769-1773 and T1771/A1775.

Conclusion

We found that the frequencies of deletion and insertion mutations in C-terminal of X-gene were more seen in cirrhotic patients comparing to chronic HBV cases in our area of study.

SUBMITTER: Salarnia F

PROVIDER: S-EPMC5427358 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Publications

Mutations in Hepatitis-B X-Gene Region: Chronic Hepatitis-B versus Cirrhosis.

Salarnia Farzaneh F Besharat Sima S Zhand Sare S Javid Naeme N Khodabakhshi Behnaz B Moradi Abdolvahab A

Journal of clinical and diagnostic research : JCDR 20170301 3

<h4>Introduction</h4>Specific mutations in Hepatitis-B Virus (HBV) genome would proceed the development of chronic hepatitis B to more serious consequences like cirrhosis and end-stage liver disease.<h4>Aim</h4>This study was designed to detect deletion and insertion mutational patterns in the X-gene region in a population of chronic HBV and related cirrhosis patients.<h4>Materials and methods</h4>Sixty eight chronic HBV patients and 34 HBV-related cirrhotics were recruited from the eligible cas ...[more]

PMID: 28511432

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Dataset Information

Mutations in Hepatitis-B X-Gene Region: Chronic Hepatitis-B versus Cirrhosis.

Introduction

Aim

Materials and methods

Results

Conclusion

Publications

Mutations in Hepatitis-B X-Gene Region: Chronic Hepatitis-B versus Cirrhosis.

Similar Datasets

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