Mutations in Hepatitis-B X-Gene Region: Chronic Hepatitis-B versus Cirrhosis.

ABSTRACT:

Introduction

Specific mutations in Hepatitis-B Virus (HBV) genome would proceed the development of chronic hepatitis B to more serious consequences like cirrhosis and end-stage liver disease.

Aim

This study was designed to detect deletion and insertion mutational patterns in the X-gene region in a population of chronic HBV and related cirrhosis patients.

Materials and methods

Sixty eight chronic HBV patients and 34 HBV-related cirrhotics were recruited from the eligible cases (N=50) referred to the academic hospitals of Gorgan city, Northeast of Iran, between Jan 2011 to Dec 2013. The HBx region was amplified by semi-nested PCR using serum samples and analyzed by sequencing.

Results

Our findings showed deletions and insertions in the C-terminal of HBx of the ci

SUBMITTER: Salarnia F 

PROVIDER: S-EPMC5427358 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature