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Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy.

ABSTRACT: The mucosal layer of conducting airways is the primary tissue exposed to inhaled microorganisms, allergens and pollutants. We developed an in vivo two-photon microscopic approach that allows performing dynamic imaging studies in the mouse trachea, which is a commonly used in vivo model of human small-diameter bronchi. By providing stabilized access to the tracheal mucosa without intubation, our setup uniquely allows dynamic in vivo imaging of mucociliary clearance and steady-state immune cell behavior within the complex airway mucosal tissue.

SUBMITTER: Veres TZ

PROVIDER: S-EPMC5429620 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy.

Veres Tibor Z TZ Kopcsányi Tamás T Tirri Marko M Braun Armin A Miyasaka Masayuki M Germain Ronald N RN Jalkanen Sirpa S Salmi Marko M

Scientific reports 20170406 1

The mucosal layer of conducting airways is the primary tissue exposed to inhaled microorganisms, allergens and pollutants. We developed an in vivo two-photon microscopic approach that allows performing dynamic imaging studies in the mouse trachea, which is a commonly used in vivo model of human small-diameter bronchi. By providing stabilized access to the tracheal mucosa without intubation, our setup uniquely allows dynamic in vivo imaging of mucociliary clearance and steady-state immune cell be ...[more]

PMID: 28386104

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