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Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo.

ABSTRACT: Understanding information processing in the brain requires monitoring neuronal activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope empowered by all-optical laser scanning, we imaged neuronal activity in vivo at up to 3,000 frames per second and submicrometer spatial resolution. This imaging method enabled monitoring of both supra- and subthreshold electrical activity down to 345??m below the brain surface in head-fixed awake mice.

SUBMITTER: Wu J

PROVIDER: S-EPMC7199528 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo.

Wu Jianglai J Liang Yajie Y Chen Shuo S Hsu Ching-Lung CL Chavarha Mariya M Evans Stephen W SW Shi Dongqing D Lin Michael Z MZ Tsia Kevin K KK Ji Na N

Nature methods 20200302 3

Understanding information processing in the brain requires monitoring neuronal activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope empowered by all-optical laser scanning, we imaged neuronal activity in vivo at up to 3,000 frames per second and submicrometer spatial resolution. This imaging method enabled monitoring of both supra- and subthreshold electrical activity down to 345 μm below the brain surface in head-fixed awake mice. ...[more]

PMID: 32123392

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