Dataset Information

The role of glutamine synthetase isozymes in enhancing nitrogen use efficiency of N-efficient winter wheat.

ABSTRACT: Glutamine synthetase (GS) isozymes play critical roles in nitrogen (N) metabolism. However, the exact relationship between GS and nitrogen use efficiency (NUE) remain unclear. We have selected and compared two wheat cultivars, YM49 and XN509, which were identified as the N-efficient and N-inefficient genotypes, respectively. In this study, agronomical, morphological, physiological and biochemical approaches were performed. The results showed that TaGS1 was high expressed post-anthesis, and TaGS2 was highly expressed pre-anthesis in N-efficient genotype compared to N-inefficient genotype. GS1 and GS2 isozymes were also separated by native-PAGE and found that the spatial and temporal distribution of GS isozymes, their expression of gene and protein subunits in source-sink-flow organs during development periods triggered the pool strength and influenced the N flow. According to the physiological role of GS isozymes, we illustrated four metabolic regulation points, by which acting collaboratively in different organs, accelerating the transport of nutrients to the grain. It suggested that the regulation of GS isozymes may promote flow strength and enhance NUE by a complex C-N metabolic mechanism. The relative activity or amount of GS1 and GS2 isozymes could be a potential marker to predict and select wheat genotypes with enhanced NUE.

SUBMITTER: Zhang Z

PROVIDER: S-EPMC5430530 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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The role of glutamine synthetase isozymes in enhancing nitrogen use efficiency of N-efficient winter wheat.

Zhang Zhiyong Z Xiong Shuping S Wei Yihao Y Meng Xiaodan X Wang Xiaochun X Ma Xinming X

Scientific reports 20170420 1

Glutamine synthetase (GS) isozymes play critical roles in nitrogen (N) metabolism. However, the exact relationship between GS and nitrogen use efficiency (NUE) remain unclear. We have selected and compared two wheat cultivars, YM49 and XN509, which were identified as the N-efficient and N-inefficient genotypes, respectively. In this study, agronomical, morphological, physiological and biochemical approaches were performed. The results showed that TaGS1 was high expressed post-anthesis, and TaGS2 ...[more]

PMID: 28428629

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Project description:Accurate and timely nitrogen (N) scheduling requires knowledge of in-season crop N deficit. Therefore, understanding the association between crop growth and crop N demand during its growth period is imperative for fine-tuning N scheduling decisions to actual crop N demand and to enhance N use efficiency. The concept of the critical N dilution curve has been employed to assess and quantify the intensity and time of crop N deficit. However, research regarding the association between crop N deficit and N use efficiency in wheat is limited. The present study was carried out to determine whether there are relationships between the accumulated nitrogen deficit (Nand) and agronomic N use efficiency (AEN) as well as with its components (N fertilizer recovery efficiency (REN) and N fertilizer physiological efficiency (PEN)) of winter wheat and to explore the potential capacity of Nand for predicting AEN and its components. Data acquired from five variable N rates (0, 75, 150, 225, and 300 kg ha−1) field experiments using six winter wheat cultivars were used to establish and validate the relationships between Nand and AEN, REN, and PEN. The results indicated that plant N concentration in winter wheat was significantly affected by N application rates. Nand varied from −65.73 to 104.37 kg ha−1 after Feekes stage 6 under different N application rates. The AEN and its components were also affected by cultivars, N levels, seasons, and growth stages. A positive correlation was observed between Nand, AEN, and its components. Validation using an independent data set showed the robustness of the newly developed empirical models to accurately predict AEN, REN, and PEN with an RMSE of 3.43 kg kg−1, 4.22%, and 3.67 kg kg−1 and RRMSE of 17.53%, 12.46%, and 13.17%, respectively. This indicates that Nand has the potential to predict AEN and its components during the growth period of winter wheat. The findings will assist in improving in-season N use efficiency by fine-tuning N scheduling decisions in winter wheat cultivation.

Dataset Information

The role of glutamine synthetase isozymes in enhancing nitrogen use efficiency of N-efficient winter wheat.

Publications

The role of glutamine synthetase isozymes in enhancing nitrogen use efficiency of N-efficient winter wheat.

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