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Integrated single- and two-photon light sheet microscopy using accelerating beams.

ABSTRACT: We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.

SUBMITTER: Piksarv P

PROVIDER: S-EPMC5431168 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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Integrated single- and two-photon light sheet microscopy using accelerating beams.

Piksarv Peeter P Marti Dominik D Le Tuan T Unterhuber Angelika A Forbes Lindsey H LH Andrews Melissa R MR Stingl Andreas A Drexler Wolfgang W Andersen Peter E PE Dholakia Kishan K

Scientific reports 20170503 1

We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range ...[more]

PMID: 28469191

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