Improved thrombolytic effect with focused ultrasound and neuroprotective agent against acute carotid artery thrombosis in rat.

ABSTRACT: Combination therapy with focused ultrasound (FUS) and a neuroprotective agent, BNG-1, was examined in an acute carotid thrombotic occlusion model using LED irradiation in rat to improve the thrombolytic effect of rt-PA. Seven treatment groups included (A) intravenous bolus injection of 0.45?mg/kg rt-PA, (B) intravenous bolus injection of 0.9?mg/kg, (C) sonothrombolysis with FUS alone, (D) oral administration of 2?g/kg BNG-1 for 7 days alone, (E) A?+?D, (F) A?+?C, and (G) A?+?C?+?D. Four comparison groups were made including (H) 0.45?mg/kg rt-PA 20% bolus +80% IV fusion?+?FUS, (I) 0.9?mg/kg rt-PA with 10% bolus?+?90% intravenous fusion, (J) B?+?C, (K) B?+?D. At 7 days after carotid occlusion, small-animal carotid ultrasound and 7?T MR angiography showed the recanalization rate of ?50% stenosis was 50% in group B and 83% in group I, but 0% in groups A and C and 17% in group D. Combination therapy improved recanalization rate to 50-63% in groups E and F, to 67-83% in groups J and K, and to 100% in groups G and H. Our study demonstrated combination therapy with different remedies can be a feasible strategy to improve the thrombolytic effect of rt-PA.

SUBMITTER: Lee TH 

PROVIDER: S-EPMC5431649 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature