Dataset Information

Eliminating interference by anthocyanin in chlorophyll estimation of sweet potato (Ipomoea batatas L.) leaves.

ABSTRACT:

Background

Spectral reflectance was evaluated for its usefulness as a nondestructive estimation of chlorophyll (Chl) content from three cultivars of sweet potato (Ipomoea batatas L.) with green, yellow, and purple leaves grown in a greenhouse for 22 days. While the green and yellow leaves contain variant amount of photosynthetic pigments without or with little level of anthocyanins, the purple leaves, except large amount of photosynthetic pigments, have high quantity of anthocyanins.

Results

For green and yellow leaves, the reciprocal reflectance (R^-1) and derived indices incorporating near infrared (NIR) reflectance, [(R_λ)^-1 - (R_NIR)^-1] and [(R_NIR/R_λ) - 1], in the green and red edge spectral ranges were shown to be strongly correlated (r² = 0.8 ~ 0.9) with the chlorophyll content. The root mean square error (RMSE) of the chlorophyll content estimation using these indices was < 50 mg m^-2. However, when purple leaves containing high levels of anthocyanins were included in the sample, R^-1 in the green spectral range and the above-mentioned indices displayed much weaker correlations with the chlorophyll content. The RMSE of chlorophyll estimation using these indices in the green spectral range sharply increased to > 110 mg m^-2 when the sample included purple leaves. The new index, [1 - (R_λ/R_NIR)], was therefore inferred and developed to eliminate the distorting effect of anthocyanins on chlorophyll content estimation using reflectance in the green spectral range. For leaves with high levels of anthocyanins, the correlation between [1 - (R_λ/R_NIR)] and the chlorophyll content remained strong (r² = 0.8 ~ 0.9) in the green spectral range, and the RMSE was minimal.

Conclusion

The reflectance index, [1 - (R_λ/R_NIR)], therefore represents a new and useful parameter for estimating leaf chlorophyll content in leaves with any level of anthocyanins such as purple rice leaf.

SUBMITTER: Huang WD

PROVIDER: S-EPMC5432896 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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Publications

Eliminating interference by anthocyanin in chlorophyll estimation of sweet potato (Ipomoea batatas L.) leaves.

Huang Wen-Dar WD Lin Kuan-Hung KH Hsu Ming-Huang MH Huang Meng-Yuan MY Yang Zhi-Wei ZW Chao Pi-Yu PY Yang Chi-Ming CM

Botanical studies 20140130 1

<h4>Background</h4>Spectral reflectance was evaluated for its usefulness as a nondestructive estimation of chlorophyll (Chl) content from three cultivars of sweet potato (Ipomoea batatas L.) with green, yellow, and purple leaves grown in a greenhouse for 22 days. While the green and yellow leaves contain variant amount of photosynthetic pigments without or with little level of anthocyanins, the purple leaves, except large amount of photosynthetic pigments, have high quantity of anthocyanins.<h4> ...[more]

PMID: 28510919

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Project description:Background and aimsThe tuberization mechanism of sweet potato (Ipomoea batatas) has long been studied using various approaches. Morphological data have revealed that the tuberizing events result from the activation of the cambium, followed by cell proliferation. However, uncertainties still remain regarding the regulators participating in this signal-transduction pathway. An attempt was made to characterize the role of one MADS-box transcription factor, which was preferentially expressed in sweet potato roots at the early tuberization stage.MethodsA differential expression level of IbMADS1 (Ipomoea batatas MADS-box 1) was detected temporally and spatially in sweet potato tissues. IbMADS1 responses to tuberization-related hormones were assessed. In order to identify the evolutionary significance, the expression pattern of IbMADS1 was surveyed in two tuber-deficient Ipomoea relatives, I. leucantha and I. trifida, and compared with sweet potato. In functional analyses, potato (Solanum tuberosum) was employed as a heterologous model. The resulting tuber morphogenesis was examined anatomically in order to address the physiological function of IbMADS1, which should act similarly in sweet potato.Key resultsIbMADS1 was preferentially expressed as tuberous root development proceeded. Its expression was inducible by tuberization-related hormones, such as jasmonic acid and cytokinins. In situ hybridization data showed that IbMADS1 transcripts were specifically distributed around immature meristematic cells within the stele and lateral root primordia. Inter-species examination indicated that IbMADS1 expression was relatively active in sweet potato roots, but undetectable in tuber-deficient Ipomoea species. IbMADS1-transformed potatoes exhibited tuber morphogenesis in the fibrous roots. The partial swellings along fibrous roots were mainly due to anomalous proliferation and differentiation in the xylem.ConclusionsBased on this study, it is proposed that IbMADS1 is an important integrator at the initiation of tuberization. As a result, the initiation and development of tuberous roots seems to be well regulated by a network involving a MADS-box gene in which such hormones as jasmonic acid and cytokinins may act as trigger factors.

Dataset Information

Eliminating interference by anthocyanin in chlorophyll estimation of sweet potato (Ipomoea batatas L.) leaves.

Background

Results

Conclusion

Publications

Eliminating interference by anthocyanin in chlorophyll estimation of sweet potato (Ipomoea batatas L.) leaves.

Similar Datasets

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