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Organic/inorganic double-layered shells for multiple cytoprotection of individual living cells.

ABSTRACT: The cytoprotection of individual living cells under in vitro and daily-life conditions is a prerequisite for various cell-based applications including cell therapy, cell-based sensors, regenerative medicine, and even the food industry. In this work, we use a cytocompatible two-step process to encapsulate Saccharomyces cerevisiae in a highly uniform nanometric (<100 nm) shell composed of organic poly(norepinephrine) and inorganic silica layers. The resulting cell-in-shell structure acquires multiple resistance against lytic enzyme, desiccation, and UV-C irradiation. In addition to the UV-C filtering effect of the double-layered shell, the biochemical responses of the encapsulated yeast are suggested to contribute to the observed UV-C tolerance. This work offers a chemical tool for cytoprotecting individual living cells under multiple stresses and also for studying biochemical behavior at the cellular level.

SUBMITTER: Hong D

PROVIDER: S-EPMC5433039 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature

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Organic/inorganic double-layered shells for multiple cytoprotection of individual living cells.

Hong Daewha D Lee Hojae H Ko Eun Hyea EH Lee Juno J Cho Hyeoncheol H Park Matthew M Yang Sung Ho SH Choi Insung S IS

Chemical science 20141001 1

The cytoprotection of individual living cells under <i>in vitro</i> and daily-life conditions is a prerequisite for various cell-based applications including cell therapy, cell-based sensors, regenerative medicine, and even the food industry. In this work, we use a cytocompatible two-step process to encapsulate <i>Saccharomyces cerevisiae</i> in a highly uniform nanometric (<100 nm) shell composed of organic poly(norepinephrine) and inorganic silica layers. The resulting cell-in-shell structure ...[more]

PMID: 28553469

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