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PCR-Dipstick Chromatography for Differential Detection of Carbapenemase Genes Directly in Stool Specimens.

ABSTRACT: A PCR-dipstick chromatography technique was designed and evaluated for differential identification of bla_NDM, bla_KPC, bla_IMP, and bla_OXA-48 carbapenemase genes directly in stool specimens within 2 h. It is a DNA-DNA hybridization-based detection system where PCR products can be easily interpreted by visual observation without electrophoresis. The PCR-dipstick showed high sensitivity (93.3%) and specificity (99.1%) in directly detecting carbapenemase genes in stool specimens compared with multiplex PCR for genomic DNA of the isolates from those stool specimens.

SUBMITTER: Shanmugakani RK

PROVIDER: S-EPMC5444138 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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PCR-Dipstick Chromatography for Differential Detection of Carbapenemase Genes Directly in Stool Specimens.

Shanmugakani Rathina Kumar RK Akeda Yukihiro Y Yamamoto Norihisa N Sakamoto Noriko N Hagiya Hideharu H Yoshida Hisao H Takeuchi Dan D Sugawara Yo Y Kodera Takuya T Kawase Mitsuo M Laolerd Warawut W Chaihongsa Narong N Santanirand Pitak P Ishii Yoshikazu Y Hamada Shigeyuki S Tomono Kazunori K

Antimicrobial agents and chemotherapy 20170524 6

A PCR-dipstick chromatography technique was designed and evaluated for differential identification of blaNDM, blaKPC, blaIMP, and blaOXA-48 carbapenemase genes directly in stool specimens within 2 h. It is a DNA-DNA hybridization-based detection system where PCR products can be easily interpreted by visual observation without electrophoresis. The PCR-dipstick showed high sensitivity (93.3%) and specificity (99.1%) in directl ...[more]

PMID: 28373197

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