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Millstone: software for multiplex microbial genome analysis and engineering.

ABSTRACT: Inexpensive DNA sequencing and advances in genome editing have made computational analysis a major rate-limiting step in adaptive laboratory evolution and microbial genome engineering. We describe Millstone, a web-based platform that automates genotype comparison and visualization for projects with up to hundreds of genomic samples. To enable iterative genome engineering, Millstone allows users to design oligonucleotide libraries and create successive versions of reference genomes. Millstone is open source and easily deployable to a cloud platform, local cluster, or desktop, making it a scalable solution for any lab.

SUBMITTER: Goodman DB

PROVIDER: S-EPMC5445467 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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Millstone: software for multiplex microbial genome analysis and engineering.

Goodman Daniel B DB Kuznetsov Gleb G Lajoie Marc J MJ Ahern Brian W BW Napolitano Michael G MG Chen Kevin Y KY Chen Changping C Church George M GM

Genome biology 20170525 1

Inexpensive DNA sequencing and advances in genome editing have made computational analysis a major rate-limiting step in adaptive laboratory evolution and microbial genome engineering. We describe Millstone, a web-based platform that automates genotype comparison and visualization for projects with up to hundreds of genomic samples. To enable iterative genome engineering, Millstone allows users to design oligonucleotide libraries and create successive versions of reference genomes. Millstone is ...[more]

PMID: 28545559

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