Ontology highlight
ABSTRACT:
SUBMITTER: Sundaram A
PROVIDER: S-EPMC5449187 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Sundaram Arunkumar A Plumb Rachel R Appathurai Suhila S Mariappan Malaiyalam M
eLife 20170515
IRE1α is an endoplasmic reticulum (ER) localized endonuclease activated by misfolded proteins in the ER. Previously, we demonstrated that IRE1α forms a complex with the Sec61 translocon, to which its substrate XBP1u mRNA is recruited for cleavage during ER stress (Plumb et al., 2015). Here, we probe IRE1α complexes in cells with blue native PAGE immunoblotting. We find that IRE1α forms a hetero-oligomeric complex with the Sec61 translocon that is activated upon ER stress with little change in th ...[more]