Unknown,Transcriptomics,Genomics,Proteomics

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Unfolded protein response in wildtype, ire1, gcn4, gcn2


ABSTRACT: We measured steady-state mRNA levels by microarray hybridization, comparing WT, (delta)ire1, (delta)gcn4, and (delta)gcn2 cells treated with 2 mM DTT for 30 min (by which time the UPR is qualitatively complete) to untreated samples of the same genotype. WT cells were taken as a positive control for UPR induction, and (delta)ire1 cells as a negative control. Fold change in expression of a given gene was computed as the ratio of mRNA level in the treated sample to the level in an untreated sample of the same genotype. Values reported here are the log2 fold change. Keywords = unfolded protein response Keywords = UPR Keywords = ire1 Keywords = gcn4 Keywords = gcn2

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Peter Walter 

PROVIDER: E-GEOD-1688 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Gcn4p and novel upstream activating sequences regulate targets of the unfolded protein response.

Patil Christopher K CK   Li Hao H   Walter Peter P  

PLoS biology 20040817 8


Eukaryotic cells respond to accumulation of unfolded proteins in the endoplasmic reticulum (ER) by activating the unfolded protein response (UPR), a signal transduction pathway that communicates between the ER and the nucleus. In yeast, a large set of UPR target genes has been experimentally determined, but the previously characterized unfolded protein response element (UPRE), an upstream activating sequence (UAS) found in the promoter of the UPR target gene KAR2, cannot account for the transcri  ...[more]

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