Ontology highlight
ABSTRACT:
SUBMITTER: Gansauge MT
PROVIDER: S-EPMC5449542 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Gansauge Marie-Theres MT Gerber Tobias T Glocke Isabelle I Korlevic Petra P Lippik Laurin L Nagel Sarah S Riehl Lara Maria LM Schmidt Anna A Meyer Matthias M
Nucleic acids research 20170601 10
DNA library preparation for high-throughput sequencing of genomic DNA usually involves ligation of adapters to double-stranded DNA fragments. However, for highly degraded DNA, especially ancient DNA, library preparation has been found to be more efficient if each of the two DNA strands are converted into library molecules separately. We present a new method for single-stranded library preparation, ssDNA2.0, which is based on single-stranded DNA ligation with T4 DNA ligase utilizing a splinter ol ...[more]