Ontology highlight
ABSTRACT:
SUBMITTER: Miura F
PROVIDER: S-EPMC6144795 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Miura Fumihito F Fujino Tomoko T Kogashi Kanako K Shibata Yukiko Y Miura Miki M Isobe Hiroyuki H Ito Takashi T
Nucleic acids research 20180901 16
Next-generation sequencing of single-stranded DNA (ssDNA) is attracting increased attention from a wide variety of research fields. Accordingly, various methods are actively being tested for the efficient adaptor-tagging of ssDNA. We conceived a novel chemo-enzymatic method termed terminal deoxynucleotidyl transferase (TdT)-assisted, copper-catalyzed azide-alkyne cycloaddition (CuAAC)-mediated ssDNA ligation (TCS ligation). In this method, TdT is used to incorporate a single 3'-azide-modified di ...[more]