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The crystal structure of Trz1, the long form RNase Z from yeast.


ABSTRACT: tRNAs are synthesized as precursor RNAs that have to undergo processing steps to become functional. Yeast Trz1 is a key endoribonuclease involved in the 3? maturation of tRNAs in all domains of life. It is a member of the ?-lactamase family of RNases, characterized by an HxHxDH sequence motif involved in coordination of catalytic Zn-ions. The RNase Z family consists of two subfamilies: the short (250-400 residues) and the long forms (about double in size). Short form RNase Z enzymes act as homodimers: one subunit embraces tRNA with a protruding arm, while the other provides the catalytic site. The long form is thought to contain two fused ?-lactamase domains within a single polypeptide. Only structures of short form RNase Z enzymes are known. Here we present the 3.1 Å crystal structure of the long-form Trz1 from Saccharomyces cerevisiae. Trz1 is organized into two ?-lactamase domains connected by a long linker. The N-terminal domain has lost its catalytic residues, but retains the long flexible arm that is important for tRNA binding, while it is the other way around in the C-terminal domain. Trz1 likely evolved from a duplication and fusion of the gene encoding the monomeric short form RNase Z.

SUBMITTER: Ma M 

PROVIDER: S-EPMC5449637 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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The crystal structure of Trz1, the long form RNase Z from yeast.

Ma Miao M   Li de la Sierra-Gallay Ines I   Lazar Noureddine N   Pellegrini Olivier O   Durand Dominique D   Marchfelder Anita A   Condon Ciarán C   van Tilbeurgh Herman H  

Nucleic acids research 20170601 10


tRNAs are synthesized as precursor RNAs that have to undergo processing steps to become functional. Yeast Trz1 is a key endoribonuclease involved in the 3΄ maturation of tRNAs in all domains of life. It is a member of the β-lactamase family of RNases, characterized by an HxHxDH sequence motif involved in coordination of catalytic Zn-ions. The RNase Z family consists of two subfamilies: the short (250-400 residues) and the long forms (about double in size). Short form RNase Z enzymes act as homod  ...[more]

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