Optimizing sample handling for urinary proteomics.
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ABSTRACT:
SUBMITTER: Lee RS
PROVIDER: S-EPMC5451898 | biostudies-literature | 2008 Sep
REPOSITORIES: biostudies-literature
Publications
Optimizing sample handling for urinary proteomics.
Lee Richard S RS Monigatti Flavio F Briscoe Andrew C AC Waldon Zachary Z Freeman Michael R MR Steen Hanno H
Journal of proteome research 20080729 9
Interrogation of the urinary proteome for clinically useful biomarkers of disease will require normalization of methods for protein extraction and sample handling. Variations in collection methods and other procedures may introduce significant discrepancies in qualitative and quantitative measurements. Here we demonstrate that the method of protein extraction, length of handling at room temperature, and repetitive freeze-thaw cycles do not seem to alter the urinary proteome at either the protein ...[more]
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