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Off-Chip-Controlled Droplet-on-Demand Method for Precise Sample Handling.

ABSTRACT: We present a simple, stable, and highly reproducible off-chip-controlled method for generating droplets-on-demand. To induce the droplet generation, externally pre-programmed positive pressure pulses are applied to the dispersed phase input while the continuous phase channel remains at constant input pressure. By controlling solely one fluid phase, the method allows for connecting multiple independent dispersed-phase channels to a single continuous channel. Experimental results show that the method allows for a droplet generation frequency of 33 Hz and a high reproducibility of droplets with standard deviations less than 5% of the mean value. Moreover, utilization of the off-chip-controlled method results in the simplicity in chip design and allows rapid (?5 min) and cost-efficient (0.5 USD) prototyping of the device.

SUBMITTER: Hamidovic M

PROVIDER: S-EPMC7203690 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Off-Chip-Controlled Droplet-on-Demand Method for Precise Sample Handling.

Hamidović Medina M Marta Uli U Bridle Helen H Hamidović Damir D Fink Gerold G Wille Robert R Springer Andreas A Haselmayr Werner W

ACS omega 20200423 17

We present a simple, stable, and highly reproducible off-chip-controlled method for generating droplets-on-demand. To induce the droplet generation, externally pre-programmed positive pressure pulses are applied to the dispersed phase input while the continuous phase channel remains at constant input pressure. By controlling solely one fluid phase, the method allows for connecting multiple independent dispersed-phase channels to a single continuous channel. Experimental results show that the met ...[more]

PMID: 32391454

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