Dataset Information

In vivo imaging of uterine cervix with a Mueller polarimetric colposcope.

ABSTRACT: Mueller polarimetric imaging enables the detection and quantification of modifications of the collagen fibers in the uterine cervix due to the development of a precancerous lesion. This information is not accessible through the use of the classic colposcope, a low magnification microscope used in current practice for cervical cancer screening. However, the in vivo application of Mueller polarimetric imaging poses an instrumental challenge: the device should be sufficiently compact, while still being able to perform fast and accurate acquisition of Mueller matrices in real-world conditions. In this study, the first wide field Mueller Polarimetric Colposcope (MPC) for the in vivo analysis of uterine cervix is presented. The MPC has been fabricated by grafting a miniaturized Mueller polarimetric imager on a classic colposcope. This new imaging tool performs the fast acquisition of Mueller polarimetric images, thus eliminating any blurring effects due to patient movements. It can be easily used by a practitioner with little change to their existing practice. Finally, the MPC was tested in vivo on a number of patients in the field.

SUBMITTER: Vizet J

PROVIDER: S-EPMC5453972 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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In vivo imaging of uterine cervix with a Mueller polarimetric colposcope.

Vizet Jérémy J Rehbinder Jean J Deby Stanislas S Roussel Stéphane S Nazac André A Soufan Ranya R Genestie Catherine C Haie-Meder Christine C Fernandez Hervé H Moreau François F Pierangelo Angelo A

Scientific reports 20170601 1

Mueller polarimetric imaging enables the detection and quantification of modifications of the collagen fibers in the uterine cervix due to the development of a precancerous lesion. This information is not accessible through the use of the classic colposcope, a low magnification microscope used in current practice for cervical cancer screening. However, the in vivo application of Mueller polarimetric imaging poses an instrumental challenge: the device should be sufficiently compact, while still b ...[more]

PMID: 28572602

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Project description:SignificanceThe polarimetric properties of biological tissues are often difficult to ascertain independent of their complex structural and organizational features. Conventional polarimetric tissue phantoms have well-characterized optical properties but are overly simplified. We demonstrate that an innovative, biologically sourced, engineered tissue construct better recapitulates the desired structural and polarimetric properties of native collagenous tissues, with the added benefit of potential tunability of the polarimetric response. We bridge the gap between non-biological polarimetric phantoms and native tissues.AimWe aim to evaluate a synthesized tissue construct for its effectiveness as a phantom that mimics the polarimetric properties in typical collagenous tissues.ApproachWe use a fibroblast-derived, ring-shaped engineered tissue construct as an innovative tissue phantom for polarimetric imaging. We perform polarimetry measurements and subsequent analysis using the Mueller matrix decomposition and Mueller matrix transformation methods. Scalar polarimetric parameters of the engineered tissue are analyzed at different time points for both a control group and for those treated with the transforming growth factor (TGF)-β1 . Second-harmonic generation (SHG) imaging and three-dimensional collagen fiber organization analysis are also applied.ResultsWe identify linear retardance and circular depolarization as the parameters that are most sensitive to the tissue culture time and the addition of TGF-β1 . Aside from a statistically significant increase over time, the behavior of linear retardance and circular depolarization indicates that the addition of TGF-β1 accelerates the growth of the engineered tissue, which is consistent with expectations. We also find through SHG images that collagen fiber organization becomes more aligned over time but is not susceptible to the addition of TGF-β1 .ConclusionsThe engineered tissue construct exhibits changes in polarimetric properties, especially linear retardance and circular depolarization, over culture time and under TGF-β1 treatments. This tissue construct has the potential to act as a controlled modular optical phantom for polarimetric-based methods.

Dataset Information

In vivo imaging of uterine cervix with a Mueller polarimetric colposcope.

Publications

In vivo imaging of uterine cervix with a Mueller polarimetric colposcope.

Similar Datasets

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