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Mueller polarimetric imaging for fast macroscopic mapping of microscopic collagen matrix remodeling by smooth muscle cells.

ABSTRACT: Smooth muscle cells (SMCs) are critical players in cardiovascular disease development and undergo complex phenotype switching during disease progression. However, SMC phenotype is difficult to assess and track in co-culture studies. To determine the contractility of SMCs embedded within collagen hydrogels, we performed polarized light imaging and subsequent analysis based on Mueller matrices. Measurements were made both in the absence and presence of endothelial cells (ECs) in order to establish the impact of EC-SMC communication on SMC contractility. The results demonstrated that Mueller polarimetric imaging is indeed an appropriate tool for assessing SMC activity which significantly modifies the hydrogel retardance in the presence of ECs. These findings are consistent with the idea that EC-SMC communication promotes a more contractile SMC phenotype. More broadly, our findings suggest that Mueller polarimetry can be a useful tool for studies of spatial heterogeneities in hydrogel remodeling by SMCs.

SUBMITTER: Chashchina O

PROVIDER: S-EPMC7960740 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Mueller polarimetric imaging for fast macroscopic mapping of microscopic collagen matrix remodeling by smooth muscle cells.

Chashchina Olga O Mezouar Hachem H Vizet Jérémy J Raoux Clothilde C Park Junha J Ramón-Lozano Clara C Schanne-Klein Marie-Claire MC Barakat Abdul I AI Pierangelo Angelo A

Scientific reports 20210315 1

Smooth muscle cells (SMCs) are critical players in cardiovascular disease development and undergo complex phenotype switching during disease progression. However, SMC phenotype is difficult to assess and track in co-culture studies. To determine the contractility of SMCs embedded within collagen hydrogels, we performed polarized light imaging and subsequent analysis based on Mueller matrices. Measurements were made both in the absence and presence of endothelial cells (ECs) in order to establish ...[more]

PMID: 33723321

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Project description:SignificanceThe polarimetric properties of biological tissues are often difficult to ascertain independent of their complex structural and organizational features. Conventional polarimetric tissue phantoms have well-characterized optical properties but are overly simplified. We demonstrate that an innovative, biologically sourced, engineered tissue construct better recapitulates the desired structural and polarimetric properties of native collagenous tissues, with the added benefit of potential tunability of the polarimetric response. We bridge the gap between non-biological polarimetric phantoms and native tissues.AimWe aim to evaluate a synthesized tissue construct for its effectiveness as a phantom that mimics the polarimetric properties in typical collagenous tissues.ApproachWe use a fibroblast-derived, ring-shaped engineered tissue construct as an innovative tissue phantom for polarimetric imaging. We perform polarimetry measurements and subsequent analysis using the Mueller matrix decomposition and Mueller matrix transformation methods. Scalar polarimetric parameters of the engineered tissue are analyzed at different time points for both a control group and for those treated with the transforming growth factor (TGF)-β1 . Second-harmonic generation (SHG) imaging and three-dimensional collagen fiber organization analysis are also applied.ResultsWe identify linear retardance and circular depolarization as the parameters that are most sensitive to the tissue culture time and the addition of TGF-β1 . Aside from a statistically significant increase over time, the behavior of linear retardance and circular depolarization indicates that the addition of TGF-β1 accelerates the growth of the engineered tissue, which is consistent with expectations. We also find through SHG images that collagen fiber organization becomes more aligned over time but is not susceptible to the addition of TGF-β1 .ConclusionsThe engineered tissue construct exhibits changes in polarimetric properties, especially linear retardance and circular depolarization, over culture time and under TGF-β1 treatments. This tissue construct has the potential to act as a controlled modular optical phantom for polarimetric-based methods.

Dataset Information

Mueller polarimetric imaging for fast macroscopic mapping of microscopic collagen matrix remodeling by smooth muscle cells.

Publications

Mueller polarimetric imaging for fast macroscopic mapping of microscopic collagen matrix remodeling by smooth muscle cells.

Similar Datasets

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