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Live Cell Imaging of Endogenous mRNA Using RNA-Based Fluorescence "Turn-On" Probe.

ABSTRACT: Messenger RNA (mRNA) plays a critical role in cellular growth and development. However, there have been limited methods available to visualize endogenous mRNA in living cells with ease. We have designed RNA-based fluorescence "turn-on" probes that target mRNA by fusing an unstable form of Spinach with target-complementary sequences. These probes have been demonstrated to be selective, stable, and capable of targeting various mRNAs for live E. coli imaging.

SUBMITTER: Ong WQ

PROVIDER: S-EPMC5460623 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Live Cell Imaging of Endogenous mRNA Using RNA-Based Fluorescence "Turn-On" Probe.

Ong Wei Qiang WQ Citron Y Rose YR Sekine Sayaka S Huang Bo B

ACS chemical biology 20161207 1

Messenger RNA (mRNA) plays a critical role in cellular growth and development. However, there have been limited methods available to visualize endogenous mRNA in living cells with ease. We have designed RNA-based fluorescence "turn-on" probes that target mRNA by fusing an unstable form of Spinach with target-complementary sequences. These probes have been demonstrated to be selective, stable, and capable of targeting various mRNAs for live E. coli imaging. ...[more]

PMID: 28103687

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