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Live imaging of mRNA using RNA-stabilized fluorogenic proteins.

ABSTRACT: Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. In this communication, fluorogenic proteins whose fluorescence is activated by RNA aptamers are described. Fluorogenic proteins are highly unstable until they bind RNA aptamers inserted into messenger RNAs, resulting in fluorescent RNA-protein complexes that enable live imaging of mRNA in living cells.

SUBMITTER: Wu J

PROVIDER: S-EPMC6719798 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Live imaging of mRNA using RNA-stabilized fluorogenic proteins.

Wu Jiahui J Zaccara Sara S Khuperkar Deepak D Kim Hyaeyeong H Tanenbaum Marvin E ME Jaffrey Samie R SR

Nature methods 20190830 9

Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. In this communication, fluorogenic proteins whose fluorescence is activated by RNA aptamers are described. Fluorogenic proteins are highly unstable until they bind RNA aptamers inserted into messenger RNAs, resulting in fluorescent RNA-protein complexes that enable live imaging of mRNA in li ...[more]

PMID: 31471614

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