Ontology highlight
ABSTRACT:
SUBMITTER: Sitarska E
PROVIDER: S-EPMC5464772 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Sitarska Ewa E Xu Junjie J Park Seungmee S Liu Xiaoxia X Quade Bradley B Stepien Karolina K Sugita Kyoko K Brautigam Chad A CA Sugita Shuzo S Rizo Josep J
eLife 20170506
Munc18-1 orchestrates SNARE complex assembly together with Munc13-1 to mediate neurotransmitter release. Munc18-1 binds to synaptobrevin, but the relevance of this interaction and its relation to Munc13 function are unclear. NMR experiments now show that Munc18-1 binds specifically and non-specifically to synaptobrevin. Specific binding is inhibited by a L348R mutation in Munc18-1 and enhanced by a D326K mutation designed to disrupt the 'furled conformation' of a Munc18-1 loop. Correspondingly, ...[more]