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A comprehensive analysis and annotation of human normal urinary proteome.


ABSTRACT: Biomarkers are measurable changes associated with the disease. Urine can reflect the changes of the body while blood is under control of the homeostatic mechanisms; thus, urine is considered an important source for early and sensitive disease biomarker discovery. A comprehensive profile of the urinary proteome will provide a basic understanding of urinary proteins. In this paper, we present an in-depth analysis of the urinary proteome based on different separation strategies, including direct one dimensional liquid chromatography-tandem mass spectrometry (LC/MS/MS), two dimensional LC/MS/MS, and gel-eluted liquid fraction entrapment electrophoresis/liquid-phase isoelectric focusing followed by two dimensional LC/MS/MS. A total of 6085 proteins were identified in healthy urine, of which 2001 were not reported in previous studies and the concentrations of 2571 proteins were estimated (spanning a magnitude of 106) with an intensity-based absolute quantification algorithm. The urinary proteins were annotated by their tissue distribution. Detailed information can be accessed at the "Human Urine Proteome Database" (www.urimarker.com/urine).

SUBMITTER: Zhao M 

PROVIDER: S-EPMC5465101 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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A comprehensive analysis and annotation of human normal urinary proteome.

Zhao Mindi M   Li Menglin M   Yang Yehong Y   Guo Zhengguang Z   Sun Ying Y   Shao Chen C   Li Mingxi M   Sun Wei W   Gao Youhe Y  

Scientific reports 20170608 1


Biomarkers are measurable changes associated with the disease. Urine can reflect the changes of the body while blood is under control of the homeostatic mechanisms; thus, urine is considered an important source for early and sensitive disease biomarker discovery. A comprehensive profile of the urinary proteome will provide a basic understanding of urinary proteins. In this paper, we present an in-depth analysis of the urinary proteome based on different separation strategies, including direct on  ...[more]

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