Ontology highlight
ABSTRACT:
SUBMITTER: Chang D
PROVIDER: S-EPMC5465217 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Chang Dingran D Tram Kha K Li Ben B Feng Qian Q Shen Zhifa Z Lee Christine H CH Salena Bruno J BJ Li Yingfu Y
Scientific reports 20170608 1
We report on a new colorimetric DNA detection method that takes advantage of the power of polymerase chain reaction (PCR) and the simplicity of the classic litmus test. The strategy makes use of a modified set of primers for PCR to facilitate ensuing manipulations of resultant DNA amplicons: their tagging with urease and immobilization onto magnetic beads. The amplicon/urease-laden beads are then used to hydrolyze urea, resulting in the increase of pH that can be conveniently reported by a pH-se ...[more]